Immunofluorescent analysis of SSEA-4 (green) showing membrane staining of NCCIT cells (right panel) compared to negative HeLa cell control (left panel). The cells were fixed with formalin for 15 minutes, washed, and then blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a DyLight 488-conjugated SSEA-4 monoclonal antibody (Product # MA1-021-D488) in 3% BSA-PBS at a dilution of 1:150 and incubated for 1 hour at 37C in the dark. F-Actin (left panel, red) was stained with DyLight 554 Phalloidin (Product # 21834) and nuclei (both panels, blue) were stained with DAPI. Images were taken at 60X magnification.
Imunofluorescent analysis of SSEA4 (green) in human episomal iPSC cells. Cells were grown on vitronectin coated plates, fixed with 4% paraformaldehyde for 15 minutes, and blocked with 3% Blocker BSA (Product # 37525) for 30 minutes at room temperature. Cells were stained with DyLight 488-conjugated SSEA4 monoclonal antibody (left panels, Product # MA1-021-D488) or DyLight 488-conjugated Mouse IgG3 isotype control antibody (top right panel, Product # MA1-190-D488), at a dilution of 1:50 and incubated for 1 hour at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on an EVOS FL Imaging system at 20X magnification.
Supplier Page from Thermo Fisher Scientific for SSEA4 Antibody DyLight 488